Plasmid

Part:BBa_K5048019:Design

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-13)


plasmid lptE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5792
    Illegal NotI site found at 5817
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5792
    Illegal BglII site found at 4964
    Illegal BamHI site found at 5786
    Illegal XhoI site found at 5826
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5792
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2263


Design Notes

full-length lptE(signal peptide included) is fused with a FLAG tag. The fusion is activated by T7 promoter.


Source

The insertion fragment is complete synthesis . The backbone is purchased from Genscript.

References